Aseptic Technique

Aseptic Technique: Types of aseptic technique

There are two main types of aseptic technique. One is the physical type which involves using instruments such as syringes or needles, while the other one is the chemical type which involves using chemicals like chlorine bleach or iodine solution.

Physical methods are used when there is no risk involved in performing them. Chemical methods involve risks when performed incorrectly and can cause infection if not done properly.

Physical Aseptic Technique (PAT)

The physical method is used when there is no risk involved in performing it. There are some precautions that need to be taken but they do not pose any significant risks.

These precautions include wearing gloves, eye protection, and protective clothing such as gowns or masks. Some of these procedures may require special equipment such as sterilization equipment or other medical supplies. Other than those things, there are no risks associated with performing the procedure itself.

Chemical Aseptic Technique (CAT)

The chemical method is used when there is a risk involved in performing it. The risks associated with the procedure are much higher than those associated with the physical method.

Chemicals used during CAT procedures can cause infections even without proper safety measures being followed. For example, using chlorine bleach will destroy many bacteria and viruses, however it can also kill off healthy cells as well causing severe damage to your body’s immune system.

Because of the risks involved, you are required to wear more personal protective equipment. You can’t just put on a lab coat, goggles, and gloves.

Instead you must wear full body protection such as a gown, mask, and gloves. The mask must be able to cover your entire face to protect your respiratory system. Goggles are also not sufficient because they do not protect your eyes from potential chemical splashes. Instead you must use a full face shield to protect your eyes.

The chemicals used during these procedures also pose the risk of combustion and explosion. You should never light a match near these chemicals or expose them to an ignition source.

Aseptic technique steps

The steps involved in performing aseptic techniques are fairly simple. The main step is to wear sterile gloves and other personal protective equipment such as eye protection, face masks, and lab coats.

After that you must locate the appropriate equipment for your specific experiment. Once you have all the equipment you need, you are ready to start working with the cultures.

The first step you take is to clean your work space with an appropriate disinfectant to ensure that your working environment is clean and free of debris or other contaminants. Once the proper safety equipment is in place, you should start to prepare the working area.

This includes laying down sheets of paper to avoid splashing or spilling any liquids.

The next step is to make sure you have all the necessary supplies and equipment laid out and ready to use. Once all of this is set up, you are ready to begin the experiment proper.

This involves following the experiment instructions carefully as described in your lab manual or other sources.

The final step of aseptic technique is to clean everything properly before leaving the lab. This step is extremely important because if any contaminants are left behind they could lead to problems the next time someone uses that equipment or works in that area.

The steps of proper aseptic technique are fairly simple and easy to follow. They can even be broken down into smaller steps to make it easier to remember everything you need to do.

Follow these steps the next time you work in the lab and you should have no problems at all.

Additional Tips

You should always wash your hands before working in the lab.

You should always wash your hands after leaving the lab.

Make sure to wash all of your equipment and materials that have come into contact with your body.

Always wear clean clothes when working in the lab. If you need to change, make sure you change in a bathroom to avoid getting clothing fibers on anything in the lab.

Make sure lab equipment, such as pipettes or flasks are clean before using them. If this is not possible, make sure you wash them before use.

Avoid letting your hair fall into lab equipment or containers.

If you must cough or sneeze, do it into your elbow and make sure to wash your hands immediately after.

Do not let children or pets enter the laboratory and stay out of the laboratory if possible if you are sick.

If working with animals or plants, make sure to wash your hands before handling any food.

Be extremely careful when removing gloves while working with anything potentially infectious. Make sure the area is clean and take them off carefully to avoid contact with anything.

When disposing of ingredients or other potentially infectious materials, double bag them and place them in a trash can with a lid. Do not let the bag touch anything outside of the container.

Sources & references used in this article:

ANTT: a standard approach to aseptic technique. by S Rowley, S Clare – Nursing times, 2011 –

ANTT v2: an updated practice framework for aseptic technique by S Rowley, S Clare, S Macqueen… – British Journal of …, 2010 –

A nursing practice problem: failure to observe aseptic technique by C McLane, S Chenelly, ML Sylwestrak… – American journal of …, 1983 – Elsevier

Aseptic technique: evidence-based approach for patient safety by RM Preston – British Journal of Nursing, 2005 –

Using an aseptic technique to reduce the risk of infection by S Hart – Nursing Standard (through 2013), 2007 –

Variations in aseptic technique and implications for infection control by A Marie Aziz – British Journal of Nursing, 2009 –

Aseptic technique for cell culture by RJ Coté – Current protocols in cell biology, 1998 – Wiley Online Library

Yes, Virginia, aseptic technique is very important: maximal barrier precautions during insertion reduce the risk of central venous catheter-related bacteremia by DG Maki – Infection Control & Hospital Epidemiology, 1994 –